Isolation and characterization of a double chain intermolecular cross-linked peptide from insoluble calf bone collagen.

Collagen cross-linking. Isolation of cross-linked peptides from collagen of chicken bone.

Cross-linked peptides were isolated from chicken bone collagen that had been digested with CNBr or with bacterial collagenase. Analyses of (3)H radioactivity in disc electrophoretic profiles of the CNBr peptides from bone collagens that had been treated with NaB(3)H indicated that a major site of intermolecular cross-linking in chicken bone collagen is located between the carboxy-terminal regio...

Octopus skin collagen. Isolation and characterization of collagen comprising two distinct alpha chains.

A major collagenous component of octopus skin was isolated from limited pepsin digests by selective salt precipitation in acidic and neutral solvents. Chromatography on Cm-cellulose of the denatured collagen and sodium dodecyl sulfate-gel electrophoresis, agarose gel filtration, and chemical analysis of the chromatographic fractions revealed that two distinct alpha chains, alpha 1 and alpha 2, ...

Isolation and Characterization of CNBr Peptides of Human [arl(III) I3 Collagen and Tissue Distribution of [al(I) 32ac2 and [arl(III) I3 Collagens”

[al(III)], collagen was solubilized by pepsin digestion of normal human placental membranes and was purified by differential salt precipitation and carboxymethylcellulose chromatography. This collagen was digested with CNBr, and the resultant nine peptides were isolated and characterized. The chains are cross-linked by cysteinyl residues in the COOH-terminal peptide. Isolation of peptides deriv...

Cross-link analysis of the C-telopeptide domain from type III collagen.

Several peptides were isolated from tryptic digests of insoluble calf aorta matrix by chromatography. Reductive pyridylethylation of a tryptic 15 kDa pool released fragments deriving from the C-terminus of type III collagen. A 50-residue peptide Tc(III) was shown by sequence analysis to be the C-terminal peptide from the alpha 1(III)-chain, containing a helical and non-helical region of equal s...

Isolation and characterization of a bovine herpesvirus 3 From cases of calf pneumonia in Iran